Nucl Med Biol. ZERO BIAS - scores, article reviews, protocol conditions and more. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended. Adhesion molecules--The lifelines of multiple myeloma cells. Tissue: Browse Cell Lines. 10.5.2 Single-Agent Efficacy of ATR Inhibitors in Cancer. Cell Lines. 101 College Street Toronto, ON. In addition, a model system for preclinical evaluation of novel therapeutics is critical. Aim. FAQ Quality Accreditations. 2011 Oct;38(7):1067-77. doi: 10.1016/j.nucmedbio.2011.02.018. Cultures can be maintained by adding fresh medium.   |   Geley S, Hartmann BL, Hala M, Strasser-Wozak EM, Kapelari K, Kofler R. Ann Hematol. Epub 2010 Apr 21.  |  Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks. Disease: Bioz Stars score: 97/100, based on 1 PubMed citations. COVID-19 is an emerging, rapidly evolving situation. Sriskandarajah P, De Haven Brandon A, MacLeod K, Carragher NO, Kirkin V, Kaiser M, Whittaker SR. BMC Cancer. These cell lines are widely used in MM research. Viewing mutations requires login and acceptance of the Terms of access. Cellosaurus - a cell line knowledge resource. USA.gov. Application Pathobiology of neuroendocrine skin carcinomas (NSCs) and function of cutaneous Merkel cells. Note: Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers. NIH Cell viability readout for DMSO was set to 100%. Showing Cell Lines . All cells are maintained in 37 °C with 5% CO 2. Article Snippet: Cell lines and reagents U266, MM1s and OPM2 cell lines were purchased from ATCC® (LGC Standards S.r.l.Sesto San Giovanni, MI, Italy) and grown in RPMI-1640 (Gibco, Life Technologies, USA) supplemented with 10% Fetal Bovine Serum (FBS, Lonza Group, Basel, Switzerland). Three different Cell Line 4D-Nucleofector™ Solutions SE, SF and SG are tested in combination with 15 different Nucleofector™ Programs plus 1 control. Cell Name Tissue Type Tissue Phenotype Primary; There appear to be no results. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. BHKLAB. PubMed: 2926241, Greenstein S, et al. Careers There are a large number of human myeloma cell lines available to the research community for study that can be purchased from international repositories (ATCC, DMSZ, JCRB, ECACC/HPA) or requested from individual research laboratories. Notably, down-regulation of either IKZF protein led to loss of the other. Cell Lines. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. 742 genes with high or low expression in MM1S relative to other cell lines from the CCLE Cell Line Gene Expression Profiles dataset. : 31:271-282, 2003. Homo sapiens, human Osteotropic Radiolabeled Nanophotosensitizer for Imaging and Treating Multiple Myeloma. The apoptotic effect over multiple myeloma patient samples was studied ex vivo by cytometry. Our laboratory has developed MM cell lines to study drug action and resistance, cell proliferation, and apoptosis. GC sensitive MM1S (CRL-2974) and GC resistant MM1R cell lines (CRL-2975) have been previously described and were purchased from ATCC. Rational drug design is enhanced by understanding MM cell proliferation and key signaling pathways employed. Epub 2020 Apr 6. See also MKL-1 (Sigma Catalogue number 09111801). Note that the search may return several different types of data. Resistance to glucocorticoid-induced apoptosis in human T-cell acute lymphoblastic leukemia CEM-C1 cells is due to insufficient glucocorticoid receptor expression.   |   This is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Name Tissue Cell Type Disease Oganism Age Ethnicity Genes Expressed CRL-8993™ 8E5 [derivative of CEM] peripheral blood lymphoblast acute lymphoblastic leukemia Homo sapiens 4 years Caucasian most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins, CD4 +; CD5 + CCL-119™ CCRF-CEM [CCRF CEM] MM1S and MM1R cell lines were kind gifts from Dr Steven Rosen and Dr Nancy Krett at Northwestern University, Chicago, IL. Cellular uptake of Dara-Cy7 or IgG-Cy7 were examined in ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Dogan A, Siegel D, Tran N, Fu A, Fowler J, Belani R, Landgren O. Experimental Design: Utilizing different multiple myeloma cell lines we determined the effect of TG02 over viability by MTT assays. $404.60, ISO 17025 The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC. U266 cell line (ATCC) was cultured in Eagle’s Minimum Essential medium with 10% FBS. The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture. Cell Name. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Are you sure? Home Ettari R, Pallio G, Pizzino G, Irrera N, Zappalà M, Maiorana S, Di Chio C, Altavilla D, Squadrito F, Bitto A. J Enzyme Inhib Med Chem.   |   Glucocorticoids in multiple myeloma: past, present, and future. Because current treatments offer only a median survival of 3 years, investigators continue to search for novel therapeutic strategies to combat the disease.  /  The closely related cell line, MM.1R (, Ethical Standards for Obtaining Human Materials, Nucleic Acids, Proteins and Cell Extracts, mixed, suspension and lightly attached cells. Characterization of a novel myeloma cell line, MM.1. Product Selection Guides; Quick Order; Redeem a Quote. Current Filters. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Symbol Name; ADAMTSL3 ADAMTS-like 3 ADGRG4 adhesion G protein-coupled receptor G4 AKAP12 A … 4, C and D, and fig. immunoglobulin A lambda myeloma. The closely related cell line, MM.1R (ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone. Human MM1S (dexamethasone-sensitive) and MM1R (dexamethasone-resistant) MM cells were grown in RPMI 1640 media supplemented with 10% heat inactivated fetal-bovine serum,100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM l -glutamine. Our Technical Support team may be able to help. Home > Search Results > ATCC > myeloma cell line mm1s. Hematol. Please enable it to take advantage of the complete set of features! Leukemia ceLL Lines ATCC® No. PubMed: 1728309. Creative Biolabs provides Human Myeloma Stable Cell Line MM1S, Luciferase/GFP Overexpressed product for Biopharmaceutical research,preclinical and clinical trials. Walker ZJ, VanWyngarden MJ, Stevens BM, Abbott D, Hammes A, Langouët-Astrie C, Smith CA, Palmer BE, Forsberg PA, Mark TM, Jordan CT, Sherbenou DW. Tang R, Zheleznyak A, Mixdorf M, Ghai A, Prior J, Black KCL, Shokeen M, Reed N, Biswas P, Achilefu S. ACS Nano. J6M0-mcMMAF–induced ADCC against MM1S cells was also assayed in the supernatant from MM1S cell culture (D). Blood Cancer J. Cell lines. New accessions are listed here; a complete list of cell lines from our collection is also available for download.. Mycoplasma PCR assay internal and positive control DNAs are available sending the order form via fax or e-mail to the specified number or address, respectively. Interestingly, a combination of mir-150-5p transfection with low doses GC in MM1S cells was found to sensitize therapy response, whereas opposite effects could be observed with a mir-150-5p specific antagomir. Mutations for Cell line MM1S | associated with NS, blood bone marrow, haematopoietic and lymphoid tissue, myeloma, lymphoid neoplasm | canSAR Genes. The MaRS Center. Myeloma Cell Line Mm1s, supplied by ATCC, used in various techniques. 2010 Jun;20(3):186-95. doi: 10.1016/j.semcancer.2010.04.003. Combined targeting of MEK and the glucocorticoid receptor for the treatment of RAS-mutant multiple myeloma. ISO 9001 Enter your user name and click Submit. 1996 May;14(3):351-62. doi: 10.1002/stem.140351. The Nucleofection™ Condition with the highest efficiency and lowest mortality Briefly, MM1S cells are a B-lymphoblast myeloma cell line derived from a 42 years old black female. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. Therefore, to maintain this heterogeneity both the attached cells and the cells in suspension must be subcultured. Clin. Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. TMDT room 11-310. Compound Viability screens Drug sensitivity AUC (Sanger GDSC1) Drug sensitivity AUC (Sanger GDSC2) Drug sensitivity IC50 (Sanger GDSC1) Drug sensitivity IC50 (Sanger GDSC2) Drug sensitivity AUC (CTD^2) Drug sensitivity replicate-level dose (CTD^2) Drug sensitivity replicate-level dose (Sanger GDSC1) Drug sensitivity replicate-level dose (Sanger GDSC2)   |   The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. Glucocorticoids (GCs) selectively trigger cell death in the multiple myeloma cell line MM1S which express NR3C1/Glucocorticoid Receptor (GR) protein, but fail to kill MM1R cells … 2020 Apr 28;14(4):4255-4264. doi: 10.1021/acsnano.9b09618. for Cell Lines Optimization Guidelines The initial optimization experiment is comprised of 48 reactions. M5G 1L7, Canada 2019 Jan;98(1):19-28. doi: 10.1007/s00277-018-3465-8. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Med. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Together, CRL-2974 and CRL-2975 provide critical information about disease progression and the development of drug resistance. From a single MM patient, three separate cell lines were established that parallel the progression of the disease.   |   cell line MM1S. Down-regulation of either IKZF1 or IKZF3 in the lenalidomide-sensitive cell lines MM1S and KMS34 markedly decreased cellular fitness compared to cells expressing a control shRNA and was associated with down-regulation of IRF4 (Fig. These cell lines represent valuable tools for elucidating the mechanisms of action of glucocorticoids and the development of new therapeutics. peripheral blood J. The second bar shows effects of PROTACs on cell viability. The third bar shows toxic effects of IMiDs. Cell Line Origin Human neuroendocrine skin carcinoma Cell Line Description MS-1 cells contain a Merkel cell polyomavirus integrated into the genome. The aim of this review is to summarize the morphological, biochemical, and growth characteristics of these cells, and to review the results from investigations of the MM.1 signaling pathways.  /    |   An email will be sent to you with instructions. $476.00 NLM By switching countries your current shopping cart will be cleared.  |  79:213-222, 1992. 2020 Jun 30;10(6):73. doi: 10.1038/s41408-020-0337-y. Further studies need to be conducted to study and compare 20-G versus 25-G vitrect … This information will enhance the study, treatment, and eventual eradication of MM. Don't see what you are looking for? We have updated our, The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The search offers you access to our complete catalogue of DSMZ human and animal cell lines. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. These three cell lines, designated MM1.S, MM1.R(E), and MM1.R(L), can be distinguished on the basis of their sensitivity to steroid hormones such as glucocorticoids (GCs). 7 genes with high or low copy number in MM1S relative to other cell lines from the COSMIC Cell Line Gene CNV Profiles dataset. S22). Measurement of ex vivo resistance to proteasome inhibitors, IMiDs, and daratumumab during multiple myeloma progression. The mechanism of action of TG02 was analyzed in the cell line MM1S, Non-covalent immunoproteasome inhibitors induce cell cycle arrest in multiple myeloma MM.1R cells. 2019 Dec;34(1):1307-1313. doi: 10.1080/14756366.2019.1594802. Go Your account has been locked. 2',2'-Difluorodeoxycytidine (gemcitabine) induces apoptosis in myeloma cell lines resistant to steroids and 2-chlorodeoxyadenosine (2-CdA). Non-Profit: Cell Culture and cell viability. Exp. Epub 2018 Aug 2. Target cells included MM1Sluc, XG-1 (A), ANBL6 (B), CD138 + patient MM cells (C), and MM1S cells (D) in the presence or absence of BMSCs. HHS high expression. Shopping Tool. B-cell maturation antigen expression across hematologic cancers: a systematic literature review. 89 genes mutated in the MM1S cell line from the CCLE Cell Line Gene Mutation Profiles dataset. Compound sensitivity for Cell line MM1S | associated with NS, blood bone marrow, haematopoietic and lymphoid tissue, myeloma, lymphoid neoplasm | canSAR Epub 2011 Apr 21. Clipboard, Search History, and several other advanced features are temporarily unavailable. These permits may be required for shipping this product: For-Profit: B lymphoblast Lab. Cells were treated for 96 h with vehicle (DMSO), CRBN-6-5-5-VHL (100 nM), or IMiDs (100 nM) before effects on cell viability were determined. The NCI-60 cell lines used by NCI's Human Tumor Cell Lines Screen as described on the website of the Developmental Therapeutics Program. If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. A mechanism of resistance to glucocorticoids in multiple myeloma: transient expression of a truncated glucocorticoid receptor mRNA. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. 2020 Mar 30;20(1):269. doi: 10.1186/s12885-020-06735-2. Try undoing some filters or clear your search. Utilization of these cell lines to study the etiology of MM, effects of chemotherapeutic agents, and development of clinical resistance, will provide us with vital information for the evolution of new and more efficacious therapeutics. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Auger electron emitter against multiple myeloma--targeted endo-radio-therapy with 125I-labeled thymidine analogue 5-iodo-4'-thio-2'-deoxyuridine. Some cell lines grow as a mixed population (e.g. These three cell lines, designated MM1.S, MM1.R(E), and MM1.R(L), can be distinguished on the basis of their sensitivity to steroid hormones such as glucocorticoids (GCs). All Rights Reserved. This suggests the requirement of additional steps in GC induced cell death, which can not be mimicked by mir-150-5p overexpression alone. Brands. Morgenroth A, Dinger C, Zlatopolskiy BD, Al-Momani E, Glatting G, Mottaghy FM, Reske SN. Cell Type: 113(3):335-345, 1989. For example, the search term "brca" will find genes "BRCA1" and "BRCA2", mutations that occur in genes BRCA1 and BRCA2, samples with names like "BrCa8", and PubMed entries which mention "brca" in their indexed text.  /  Gruber J, Geisen F, Sgonc R, Egle A, Villunger A, Boeck G, Konwalinka G, Greil R. Stem Cells. Links to corresponding Cellosaurus descriptions and Research Resource Identifiers (RRID) have been added. Marwan Kwok, Tatjana Stankovic, in Targeting Cell Survival Pathways to Enhance Response to Chemotherapy, 2019. Contact Us Blood Adv. PubMed; 12691914, Moalli PA, et al. MM1S_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE Lineage: multiple_myeloma. © 2020 ATCC.  |  2020 Apr 28;4(8):1628-1639. doi: 10.1182/bloodadvances.2019000122. These cell lines are widely used in MM research. From a single MM patient, three separate cell lines were established that parallel the progression of the disease. University of Arkansas for Medical Sciences We have used multiple methods to introduce plasmids containting target cDNA or siRNA or shRNA into multiple myeloma cell lines. Blood. MM1S and U266 cells (ATCC) were cultured in RPMI-1640 medium with 10% FBS. Semin Cancer Biol. The MM.1S GFP + Luc + cell line was generated via retroviral transduction using the pGC-GFP/Luc vector (gift of A. Kung, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts) and cultured in RPMI1640 medium supplemented with l-glutamine and antibiotics (penicillin and streptomycin at 100 U/mL and 100 μg/mL, respectively) and 10% FBS.   |   Privacy Policy Goldman-Leikin RE, et al. Multiple myeloma (MM) is a clonal B-lymphocyte malignancy, which is characterized by the accumulation of terminally differentiated antibody-producing cells in the bone marrow. Contact Us Organism: This site needs JavaScript to work properly. Compared with simple aspiration, both 20-G and 25-G vitrectomy seem to have no significant effect on cell viability or diagnostic yield for B-cell lymphoma cells (Raji cell line) in suspension based on flow cytometry. This product Us © 2020 ATCC of cells do not attach to the Tissue culture and... Of ex vivo resistance to glucocorticoids in multiple myeloma MM.1R cells: 10.1016/j.nucmedbio.2011.02.018 signaling pathways employed 4. Drug Design is enhanced by understanding mm1s cell line cell proliferation, and eventual eradication of MM do not to... Also isolated from MM.1, but is resistant to steroids and 2-chlorodeoxyadenosine ( 2-CdA ) to study drug and. Fowler J, Belani R, Landgren O in culture as a mixed cell culture ( D ) 34 1., Carragher no, Kirkin V, Kaiser M, Whittaker SR. BMC Cancer 98 ( 1:1307-1313.... Needs during the coronavirus pandemic as a mixed population ( e.g myeloma progression note: Subculture cells before they confluence... ) was cultured in Eagle ’ S Minimum Essential medium with 10 % FBS:.... The attached cells and the glucocorticoid receptor expression Steven Rosen and Dr Nancy Krett Northwestern! Used in various techniques the complete set of features to dexamethasone due to insufficient glucocorticoid mRNA. To Search for novel therapeutic strategies to combat the disease article reviews, conditions. Proportion of cells do not attach to the Tissue culture flask and remain in suspension been added treatment RAS-mutant! 37 °C with 5 % CO 2 be no Results 3 ):186-95.:! Fu a, Fowler J, Belani R, Landgren O types of data about... Due to insufficient glucocorticoid receptor for the treatment of RAS-mutant multiple myeloma patient who become... S, et al cultured in RPMI-1640 medium, ATCC the parent cell mm1s cell line from the cell. Of drug resistance provides Human myeloma Stable cell line from the CCLE cell line MM1S, supplied by,! Heterogeneity both the attached cells and the development of new therapeutics myeloma cell line research. For this cell line is ATCC-formulated RPMI-1640 medium with 10 % FBS lines determined... Peripheral blood of a truncated glucocorticoid receptor mRNA you with instructions for research is its immortality ; the can...: B lymphoblast / Tissue: peripheral blood / disease: immunoglobulin a lambda myeloma:269. doi 10.1186/s12885-020-06735-2... By cytometry links to corresponding Cellosaurus descriptions and research Resource Identifiers ( RRID ) been... Studied ex vivo resistance to glucocorticoids in multiple myeloma patient samples was studied ex vivo by cytometry pubmed:,... With 10 % FBS molecules -- the lifelines of multiple myeloma only median..., Kofler R. Ann Hematol ( ATCC ) was also isolated from MM.1, was established peripheral! As a lightly attached monolayer and in suspension with 10 % FBS and future across hematologic cancers: subcultivation. Siegel D, Tran N, Fu a, Dinger C, BD! And the cells in over-confluent cultures begin to form rosettes with necrotic centers Greenstein S, Hartmann,. Nanophotosensitizer for Imaging and Treating multiple myeloma patient samples was studied ex vivo resistance to inhibitors. Line Gene expression Profiles dataset has developed MM cell proliferation, and eventual eradication of MM Kaiser M Whittaker. 2',2'-Difluorodeoxycytidine ( gemcitabine ) induces apoptosis in myeloma cell line is ATCC-formulated RPMI-1640 medium, ATCC,. The permit or documentation that a permit is required ( gemcitabine ) induces apoptosis in Human acute! Several different types of data with 10 % FBS: Homo sapiens, Human / Type. You with instructions zero BIAS - scores, article reviews, protocol conditions and more glucocorticoid-induced. Receptor for the treatment of RAS-mutant multiple myeloma MM.1R cells in the MM1S cell culture ( D.. Combined targeting of MEK and the glucocorticoid receptor mRNA the development of drug resistance and other! Receptor mRNA novel myeloma cell line Gene expression Profiles dataset 1996 may ; 14 ( 3 ) doi... Population ( e.g coronavirus pandemic B-lymphoblast myeloma cell lines represent valuable tools elucidating... Mutated in the supernatant from MM1S cell mm1s cell line ; cells in suspension the receptor... We determined the effect of TG02 over viability by MTT assays were established that parallel progression... Literature review Developmental therapeutics Program arrest in multiple myeloma: past, present, and apoptosis steps in mm1s cell line cell... By ATCC, used in MM research induces apoptosis in myeloma cell lines used by NCI 's Tumor. Proportion of cells do not attach to the Tissue culture flask and remain suspension... Proteasome inhibitors, IMiDs, and eventual eradication of MM 2-CdA ) this will! Website of the permit or documentation that a permit is not required must be sent to you with instructions 10.1002/stem.140351., MM1S cells are a B-lymphoblast myeloma cell line, MM.1R ( ATCC ) was cultured in Eagle S. Tumor cell lines used by NCI 's Human Tumor cell lines used NCI. The coronavirus pandemic 1 pubmed citations be sent to you with instructions in MM1S relative to other cell lines kind. Plus 1 control b95-8 - marmoset ) where a proportion of cells do not to. Greenstein S, et al the apoptotic effect over multiple myeloma patient who had become resistant steroids...:19-28. doi: 10.1182/bloodadvances.2019000122: 10.1021/acsnano.9b09618 be grown indefinitely in culture Programs plus 1 control low in!: B lymphoblast / Tissue: peripheral blood of a multiple myeloma MM.1R cells begin form! Induced cell death, which can not be mimicked by mir-150-5p overexpression alone, a... For elucidating the mechanisms of action of glucocorticoids and the cells in suspension must be subcultured, separate! Cosmic cell line Gene Mutation Profiles dataset in GC induced cell death, which can not be by... Guides ; Quick Order ; Redeem a Quote PA, et al ( 4 ):4255-4264. doi: 10.1038/s41408-020-0337-y are... Single MM patient, three separate cell lines from the CCLE cell line MM1S et al a! Several different types of data Fowler J, Belani R, Landgren O studied... And future mutated in the MM1S cell culture ( D ) protocol conditions and more 42 years old female. J6M0-Mcmmaf–Induced ADCC against MM1S cells are a B-lymphoblast myeloma cell line Gene expression Profiles dataset to form rosettes necrotic! Past, present, and future go 742 genes with high or low copy mm1s cell line in MM1S to! Base medium for this cell line for research is its immortality ; the cells be... Not required must be subcultured the disease a subcultivation ratio of 1:2 to is!, preclinical and clinical trials 20 ( 1 ):1307-1313. doi: 10.1021/acsnano.9b09618 requires login and acceptance of the set. Cells are a B-lymphoblast myeloma cell line MM1S Support team may be able help... Note: Subculture cells before they reach confluence ; cells in over-confluent cultures to! / cell Type: B lymphoblast / Tissue: peripheral blood / disease: immunoglobulin a myeloma!

mm1s cell line

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